Functional deficiencies of granulocyte-macrophage colony stimulating factor and interleukin-3 contribute to insulitis and destruction of beta cells.

Functional Deficiencies of Granulocyte-Macrophage Colony Stimulating Factor and Interleukin-3 Contribute to Insulitis and Destruction of β-Cells Short title: GM-CSF, IL-3 and Diabetes

The pathogenesis of type I diabetes (T1D) involves the immune-mediated destruction of insulin producing β-cells in the pancreatic islets of Langerhans. Genetic analysis of families with a high incidence of T1D and non-obese diabetic (NOD) mice, a prototypical model of the disorder, uncovered multiple susceptibility loci, although most of the underlying immune defects remain to be delineated. He...

Functional deficiencies of granulocyte-macrophage colony stimulating factor and interleukin-3 contribute to insulitis and destruction of cells

1Department of Medical Oncology, Dana-Farber Cancer Institute and Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA; 2Howard Hughes Medical Institute and Program in Immunology, Memorial Sloan-Kettering Cancer Center, New York, NY; 3Department of Biostatistics and Computational Biology, Dana-Farber Cancer Institute and Department of Medicine, Brigham and...

IMMUNOBIOLOGY Functional deficiencies of granulocyte-macrophage colony stimulating factor and interleukin-3 contribute to insulitis and destruction of cells

1Department of Medical Oncology, Dana-Farber Cancer Institute and Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA; 2Howard Hughes Medical Institute and Program in Immunology, Memorial Sloan-Kettering Cancer Center, New York, NY; 3Department of Biostatistics and Computational Biology, Dana-Farber Cancer Institute and Department of Medicine, Brigham and...

Comparison of T7- and Lac-Based Systems for the Periplasmic Expression of Human Granulocyte Macrophage Colony Stimulating Factor in Escherichia coli

Expression and Secretion of Human Granulocyte Macrophage-Colony Stimulating Factor Using Escherichia coli Enterotoxin I Signal Sequence

With the aim of the secretion of human granulocyte macrophage-colony stimulating factor (hGM-CSF) in Escherichia coli, hGM-CSF cDNA was fused in-frame next to the signal sequence of ST toxin (ST-I) of exteroxigenic E. coli, containing 53 or 19 amino acids of signal peptide. The fused STsig::hGM-CSF coding fragments were inserted into a T7-based expression plasmid. The recombinant plasmids were ...